Abstract: Objective To investigale the expression and its clinical significance of p53 and EGFR in gastric cancer.Methods The expressions of p53 and EGFR were detected in 45 cases normal gastric mucosa and 320 cases of gastric cancer by immunohistochemistry of PV-6000.Results The positive expression rates of P53 and EGFR in 320 cases of gastric cancer were significantly higher than those in normal gastric mucosa.There were statistically significant between the tissues of normal gastric mucosa and gastric cancer(P＜0.01).In 320 cases of gastric cancer,p53 protein expression was related to gastric cancer differentiation,stomach infiltration depth and lymph node metastasis.The expression of EGFR was related to gastric cancer differentiation,tumor size,stomach infiltration depth and lymph node metastasis.Conclusion p53 and EGFR play a certain role in the development,progression of gastric cancer.The experiment may provide a theoretical foundation for clinical therapy of gastric cancer.