Abstract: Objective To investigate the applicative value of transvaginal color doppler sonography combined with CA125 which is tumor detection maker in the the diagnosis of ovarian of benign and malignant tumor.Methods We analyzed sonographic features,blood flow of transvaginal color doppler sonography and serum CA125 level in 102 ovarian tumor cases and compared with pathological findings and study the sensitivity,specificity and accuracy of diagnosis.Results The sensitivity,specificity and accuracy of the diagnosis of ovarian of benign and malignant tumor detected by transvaginal color doppler sonography were respectively 80.6%,95.8% and 91.2%.The sensitivity,specificity and accuracy of the diagnosis of ovarian of benign and malignant tumor detected by tumor makers CA125 were 87.1%,87.3% and 87.3% respectively.The sensitivity,specificity and accuracy of the diagnosis of ovarian of benign and malignant detected by transvaginal color doppler sonography combined with tumor makers CA125 were 93.5%,97.2% and 96.1% respectively.The sensitivity,specificity and accuracy of results detected by the combination of both methods were higher than the single one.Conclusion The combination among the sonographic features,blood flow of transvaginal color doppler sonography and serum tumor markers CA125 improves the efficiency of ovarian benign and malignant tumor diagnosis,and therefore shows the great clinical value.