Abstract: Objective The tumor model was built with different chemical inducers in green fluorescent protein transfected C57BL/6(GFP-C57BL/6)mouse,and the sensitivity and efficiency were analysis.Methods In this research,benzo(α)pyrene,3-methylcholanthrene,6-nitrobiphenyl and sacrosine ethyl nitrosamine were used to induce forestomach neoplasia in GFP-C57BL/6 mouse respectively.Results The four inducers can be induced forestomach neoplasia successfully.Benzo(α)pyrene was more effective that the tumor incidence reached 100%,and it did not lead to stomach injury of its control mice.Conclusion Benzo(α)pyrene could be used for a inducer in GFP-C57BL/6 forestomach neoplasia model.