LncRNA SOX2OT海绵吸附miR-34a促进SOX2表达增强大肠癌多药耐药

doi:10.11904/j.issn.1002-3070.2020.05.003

实用肿瘤学杂志 ›› 2020, Vol. 34 ›› Issue (5): 398-403.doi: 10.11904/j.issn.1002-3070.2020.05.003

LncRNA SOX2OT海绵吸附miR-34a促进SOX2表达增强大肠癌多药耐药

郭飘婷¹, 邹阳²

1.浙江中医药大学附属第二医院全科医学科(杭州 310005);
2.浙江中医药大学附属第二医院骨科

收稿日期:2020-04-04 出版日期:2020-10-28 发布日期:2020-11-02
通讯作者: 郭飘婷,E-mail:522135842@qq.com
作者简介:郭飘婷,女,(1990-),硕士,主治医师,从事中西医结合抗肿瘤相关的研究。
基金资助:
国家自然科学基金青年项目(编号:81803876)

LncRNA SOX2OT sponge adsorbs miR-34a to promote SOX2 expression and enhance multidrug resistance in colorectal cancer

GUO Piaoting¹, ZOU Yang²

1. Department of General Medicine,The Second Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine,Hangzhou 310005,China;
2. Department of Orthopedics,The Second Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine

Received:2020-04-04 Online:2020-10-28 Published:2020-11-02

摘要/Abstract

摘要： 目的验证长链非编码RNA SOX2OT(Long noncoding RNA SOX2OT,SOX2OT)增强大肠癌HCT-116细胞多药耐药(Multidrug resistance,MDR),并从SOX2OT/miR-34a/SOX2信号通路探讨其可能的有效机制。方法双荧光素酶报告基因验证SOX2OT与miR-34a、miR-34a与SOX2的靶向结合;HCT-116细胞瞬时转染SOX2OT质粒、miR-34a mimic,RT-qPCR验证转染效率;RT-qPCR检测SOX2OT、miR-34a和SOX2表达水平并进行相关性分析;CCK-8检测细胞增殖,评估SOX2OT、miR-34a对HCT-116细胞5-氟尿嘧啶(5-FU)、长春新碱(VCR)、顺铂(CDDP)、紫杉醇(TAXOL)化疗敏感性的影响。结果 SOX2OT与miR-34a、miR-34a与SOX2之间存在靶向关系;SOX2OT抑制miR-34a(P＜0.05),上调SOX2(P＜0.01);miR-34下调SOX2OT(P＜0.01)和SOX2(P＜0.01);转染SOX2OT质粒后HCT-116细胞的5-FU、VCR、CDDP、TAXOL的IC₅₀值均升高(P＜0.01);HCT-116细胞共转染SOX2OT、miR-34a mimic时对四种化疗药物的IC₅₀值与转染SOX2OT组相比均明显降低(P＜0.01),其中5-FU、CDDP的IC₅₀值与对照组相比无统计学差异(P＞0.05)。结论 SOX2OT海绵吸附miR-34a促进SOX2的表达致使HCT-116细胞多药耐药。

关键词: 大肠癌, 多药耐药, SOX2OT, miR-34a, SOX2

Abstract: Objective The aim of this study was to verify that long noncoding RNA SOX2OT(SOX2OT)enhanced the multidrug resistance(MDR)of colorectal cancer HCT-116 cells,and explored its possible effective mechanism on SOX2OT/miR-34a/SOX2 signaling pathway.Methods Dual luciferase reporter gene assay was used to verify the targeted binding of SOX2OT to miR-34a,and miR-34a to SOX2;The SOX2OT plasmid and miR-34a were transient transfected into HCT-116 cells,and the transfection efficiency was verified by RT-qPCR;RT-qPCR was used to detect the levels of SOX2OT,miR-34a and SOX2,and the correlation amongst them were analyzed;The CCK-8 assay was used to detect cell proliferation and to assess the influence of SOX2OT and miR-34a on the chemosensitivity of HCT-116 cells to 5-fluorouracil(5-FU),vincristine(VCR),cisplatin(CDDP),and paclitaxel(Taxol).Results There was a targeting relationship between SOX2OT and miR-34a,and miR-34a with SOX2;The SOX2OT inhibited miR-34a(P＜0.05)and upregulated the level of SOX2(P＜0.01);The MiR-34a down-regulated the expression of SOX2OT and SOX2(P＜0.01);The IC₅₀ values for 5-FU,VCR,CDDP,and TAXOL in HCT-116 cells were increased after transfection of SOX2OT plasmid(P＜0.01);The IC₅₀ values of four chemotherapy drugs in the co-transfected with SOX2OT and miR-34a mimic groups were significantly lower than those of the transfected SOX2OT group(P＜0.01).The IC₅₀ values of 5-FU and CDDP were not different from the control group(P＞0.05).Conclusion The SOX2OT sponge adsorbs miR-34a and promotes the expression of SOX2,resulting in multidrug resistance of HCT-116 cells.

Key words: Colorectal cancer, Multidrug resistance, SOX2OT, miR-34a

中图分类号:

R735.3⁺4

郭飘婷, 邹阳. LncRNA SOX2OT海绵吸附miR-34a促进SOX2表达增强大肠癌多药耐药[J]. 实用肿瘤学杂志, 2020, 34(5): 398-403.

GUO Piaoting, ZOU Yang. LncRNA SOX2OT sponge adsorbs miR-34a to promote SOX2 expression and enhance multidrug resistance in colorectal cancer[J]. Journal of Practical Oncology, 2020, 34(5): 398-403.

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http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/Y2020/V34/I5/398

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LncRNA SOX2OT海绵吸附miR-34a促进SOX2表达增强大肠癌多药耐药

LncRNA SOX2OT sponge adsorbs miR-34a to promote SOX2 expression and enhance multidrug resistance in colorectal cancer

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